Mona Lisa is the subject of the famous painting that came to life when the planets aligned. She appears in the episode So in Louvre are we two.
- The "Mona Lisa" is a portrait by the Italian artist Leonardo Da Vinci.
- In The Transplant, Courage painted the Mona Lisa as part of a contest with Eustace.
- Unlike most of the animated art characters in the museum, like The Thinker, she speaks with an accent, which is Italian. This is because da Vinci actually painted the iconic portrait from a real life Italian woman identified as Lisa Gherardini (a.k.a. Lisa del Giacondo).